Sorbonne University, UPMC University of Paris 6

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

In Vitro Differentiation of Mature Myofibers for Live Imaging

Mafalda R. Pimentel¹, Sestina Falcone², Bruno Cadot², Edgar R. Gomes^1,2

¹Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, ²Myology Research Center, UM76-INSERM U974-CNRS FRE 361, Sorbonne University, UPMC University of Paris 6

Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.